Site-specific mutation of Staphylococcus aureus VraS reveals a crucial role for the VraR-VraS sensor in the emergence of glycopeptide resistance -- Galbusera et al., 10.1128/AAC.00720-10 -- Antimicrobial Agents and Chemotherapy.
Transcriptional analysis of vraS-H156A strains revealed a pronounced reduction but not complete abrogation of the vraRS operon following exposure to cell wall active antibiotics suggesting that additional factors independent of VraS-driven phosphotransfer, or {sigma}B, exist for this promoter. Collectively, our results reveal important details of the VraRS signaling system and predict that pharmacologic blockade of the VraS sensor kinase will have profound effects on blocking emergence of cell wall active antibiotic resistance in S. aureus.
